zFactor {synlet}R Documentation

Calcualte the Z and Z' factor

Description

calcualte the Z and Z' factor for each plate.

Usage

zFactor(dat, negativeCon, positiveCon, useMean = TRUE)

Arguments

dat

synthetic lethal RNAi screen data.

negativeCon

the negative control used in the WELL_CONTENT_NAME.

positiveCon

the positive control used in the WELL_CONTENT_NAME.

useMean

use mean to calcualate z factor and z' factor by default; otherwise use median.

Value

A data.frame contains z factor and z' factor

References

Zhang J.H., Chung T.D. & Oldenburg K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. B, 4 67-73 (1999). Birmingham,A. et al. (2009) Statistical methods for analysis of high-throughput RNA interference screens. Nat Methods, 6, 569-575.

Examples

zFactor(exampleDat, negativeCon = "scrambled control si1", positiveCon = "PLK1 si1")

[Package synlet version 1.10.0 Index]