NanoString nCounter data are gene expression assays where there is no need for the use of enzymes or amplification protocols and work with fluorescent barcodes (Geiss et al. (2018) <doi:10.1038/nbt1385>). Each barcode is assigned a messenger-RNA/micro-RNA (mRNA/miRNA) which after bonding with its target can be counted. As a result each count of a specific barcode represents the presence of its target mRNA/miRNA. 'NACHO' (NAnoString quality Control dasHbOard) is able to analyse the exported NanoString nCounter data and facilitates the user in performing a quality control. 'NACHO' does this by visualising quality control metrics, expression of control genes, principal components and sample specific size factors in an interactive web application.
| Version: | 2.0.6 |
| Depends: | R (≥ 3.6.0) |
| Imports: | utils, data.table, ggplot2 (≥ 3.3.0), ggforce (≥ 0.3.1), ggrepel (≥ 0.8.1), knitr (≥ 1.25), rmarkdown (≥ 1.16), shiny (≥ 1.4.0), shinyWidgets (≥ 0.4.9) |
| Suggests: | roxygen2 (≥ 7.2.0), testthat (≥ 2.2.1), covr (≥ 3.3.2), Biobase, GEOquery, limma |
| Published: | 2024-01-12 |
| DOI: | 10.32614/CRAN.package.NACHO |
| Author: | Mickaël Canouil |
| Maintainer: | Mickaël Canouil <pro at mickael.canouil.dev> |
| BugReports: | https://github.com/mcanouil/NACHO/issues |
| License: | GPL-3 |
| URL: | https://github.com/mcanouil/NACHO/, https://m.canouil.dev/NACHO/ |
| NeedsCompilation: | no |
| SystemRequirements: | pandoc (>= 1.14), pandoc-citeproc |
| Citation: | NACHO citation info |
| Materials: | README, NEWS |
| In views: | Omics |
| CRAN checks: | NACHO results |
| Reference manual: | NACHO.html , NACHO.pdf |
| Vignettes: |
NACHO-analysis (source, R code) NACHO (source, R code) |
| Package source: | NACHO_2.0.6.tar.gz |
| Windows binaries: | r-devel: NACHO_2.0.6.zip, r-release: NACHO_2.0.6.zip, r-oldrel: NACHO_2.0.6.zip |
| macOS binaries: | r-release (arm64): NACHO_2.0.6.tgz, r-oldrel (arm64): NACHO_2.0.6.tgz, r-release (x86_64): NACHO_2.0.6.tgz, r-oldrel (x86_64): NACHO_2.0.6.tgz |
| Old sources: | NACHO archive |
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