Package: sisti
Title: Real-Time PCR Data Sets by Sisti et al. (2010)
Version: 0.0.1
Authors@R: c(
    person("Ramiro", "Magno", , "rmagno@pattern.institute", role = c("aut", "cre"),
           comment = c(ORCID = "0000-0001-5226-3441")),
    person("Pattern Institute", role = c("cph", "fnd"))
  )
Description: This data package contains four datasets of quantitative PCR (qPCR)
  amplification curves that were used as supplementary data in the research
  article by Sisti et al. (2010), <doi:10.1186/1471-2105-11-186>.
  The primary dataset comprises a ten-fold dilution series spanning
  copy numbers from 3.14 × 10^7 to 3.14 × 10^2, with twelve replicates per
  concentration. These samples are based on a pGEM-T Promega plasmid containing
  a 104 bp fragment of the mitochondrial gene NADH dehydrogenase 1 (MT-ND1),
  amplified using the ND1/ND2 primer pair. The remaining three datasets contain
  qPCR results in the presence of specific PCR inhibitors: tannic acid,
  immunoglobulin G (IgG), and quercetin, respectively, to assess their effects
  on the amplification process. These datasets are useful for researchers 
  interested in PCR kinetics. The original raw data file is available as
  Additional File 1:
  <https://static-content.springer.com/esm/art%3A10.1186%2F1471-2105-11-186/MediaObjects/12859_2009_3643_MOESM1_ESM.XLS>.
License: CC BY 4.0
Encoding: UTF-8
RoxygenNote: 7.3.1
Imports: tibble
Depends: R (>= 2.10)
LazyData: true
URL: https://rmagno.eu/sisti/, https://github.com/ramiromagno/sisti
BugReports: https://github.com/ramiromagno/sisti/issues
NeedsCompilation: no
Packaged: 2024-04-28 02:24:37 UTC; rmagno
Author: Ramiro Magno [aut, cre] (<https://orcid.org/0000-0001-5226-3441>),
  Pattern Institute [cph, fnd]
Maintainer: Ramiro Magno <rmagno@pattern.institute>
Repository: CRAN
Date/Publication: 2024-04-28 11:00:02 UTC
Built: R 4.4.3; ; 2025-10-21 13:56:00 UTC; windows
